Faculty Collaboration Database

Immunopathologic responses to Aspergillus antigen in interleukin-4 knockout mice. J Lab Clin Med 1997 Dec;130(6):567-75

Date

01/09/1998

Pubmed ID

9422330

DOI

10.1016/s0022-2143(97)90106-2

Scopus ID

2-s2.0-0031460544 (requires institutional sign-in at Scopus site)   35 Citations

Abstract

Two strains of interleukin-4 (IL-4) gene knockout mice were studied and compared with wild strains to determine the role of IL-4 in the immunopathogenesis of murine allergic aspergillosis. Animals immunized intraperitoneally were subsequently challenged with Aspergillus antigen intranasally. The animals were evaluated for total serum immunoglobulin E (IgE) levels, Aspergillus-specific IgG antibody isotypes, peripheral blood eosinophils, cytokine and chemokine mRNA transcripts in spleen cells, and pulmonary histology. No serum IgE was detected in animals deficient in the IL-4 gene. Aspergillus-specific IgG1 was detected in all animals, while enhanced levels of IgG2a were detected in IL-4 knockout animals challenged with A. fumigatus antigen. There were no differences in the peripheral blood or lung eosinophils in the two groups of mice exposed to A. fumigatus. These results indicate that lung injury in Aspergillus-antigen challenged animals may be the result of the eosinophil mediators and that IgE-mediated injury may not be significant in this model, which may be a significant variation between the model and human allergic aspergillosis.

Author List

Kurup VP, Guo J, Murali PS, Choi H, Fink JN

MESH terms used to index this publication - Major topics in bold

Animals
Antibodies, Fungal
Antigens, Fungal
Aspergillosis, Allergic Bronchopulmonary
Aspergillus fumigatus
Bone Marrow
Chemokines
Cytokines
Eosinophils
Immunoglobulin E
Immunoglobulin G
Interleukin-4
Mice
Mice, Inbred C57BL
Mice, Knockout
Peroxidase
Spleen
Transcription, Genetic