Topical treatment with black raspberry extract reduces cutaneous UVB-induced carcinogenesis and inflammation. Cancer Prev Res (Phila) 2009 Jul;2(7):665-72
Date
07/09/2009Pubmed ID
19584078Pubmed Central ID
PMC3874934DOI
10.1158/1940-6207.CAPR-08-0193Scopus ID
2-s2.0-69249188386 (requires institutional sign-in at Scopus site) 64 CitationsAbstract
Light in the UVB spectrum (280-320 nm) induces a number of changes in the epidermis and dermis of mice and humans, resulting in a robust inflammatory response. A standardized black raspberry extract (BRE) has been effective in reducing signaling pathways commonly initiated by inflammatory stimuli. In this study, we determined whether this extract could reduce cutaneous UVB-induced inflammation and carcinogenesis. In our carcinogenesis model, female SKH-1 hairless mice were exposed to one minimal erythemal dose of UVB thrice weekly on nonconsecutive days for 25 weeks. Immediately after each exposure, the mice were treated topically with either BRE dissolved in vehicle or with vehicle only. Beginning on week 19, mice treated with BRE had a significant reduction in tumor number and in average tumor size. This reduction correlated with a significant reduction in tumor-infiltrating CD3(+)foxp3(+) regulatory T-cells. In the acute model, mice were exposed to a single minimal erythemal dose of UVB and treated topically with BRE or with vehicle. At 48 hours post-UVB exposure, topical BRE treatment significantly reduced edema, p53 protein levels, oxidative DNA damage, and neutrophil activation. The ability of topical BRE to reduce acute UVB-induced inflammation and to decrease tumor development in a long-term model provides compelling evidence to explore the clinical efficacy of BRE in the prevention of human skin cancers.
Author List
Duncan FJ, Martin JR, Wulff BC, Stoner GD, Tober KL, Oberyszyn TM, Kusewitt DF, Van Buskirk AMMESH terms used to index this publication - Major topics in bold
Administration, TopicalAnimals
CD3 Complex
Carcinogens
Carcinoma, Squamous Cell
DNA Damage
Female
Fruit
Inflammation
Mice
Neoplasms
Neoplasms, Radiation-Induced
Neutrophils
Plant Extracts
T-Lymphocytes, Regulatory
Tumor Suppressor Protein p53
Ultraviolet Rays
