Effect of cortisol and urea on flavin monooxygenase activity and expression in rainbow trout, Oncorhynchus mykiss. Mar Environ Res 2002;54(3-5):275-8
Date
11/01/2002Pubmed ID
12408576DOI
10.1016/s0141-1136(02)00183-6Scopus ID
2-s2.0-0036753537 (requires institutional sign-in at Scopus site) 13 CitationsAbstract
Expression of flavin-containing monooxygenase(s) (FMO) correlates with salinity exposure in certain species of euryhaline fish, such as the rainbow trout, Oncorhynchus mykiss. The mechanism(s) by which salinity regulates FMO is unclear. Adult rainbow trout were infused through the dorsal aorta with either cortisol or urea. At 500 ng/ml, cortisol caused a significant increase in FMO-catalyzed thiourea oxidase activity in gill and liver microsomes. FMOI expression, however, was significantly increased by the high cortisol dose only in gill microsomes. The levels of TMAO and urea were not altered by cortisol. In the liver, urea infusion caused an increase in hepatic FMO activity. FMO expression and activity correlated with elevated tissue urea levels, but TMAO concentrations were not related. These results indicate that FMO expression and activity may be partially controlled by the osmoregulatory/stress hormone. cortisol, and concentrations of the organic osmolyte, urea, in the rainbow trout.
Author List
El-Alfy A, Larsen B, Schlenk DAuthor
Abir El-Alfy PhD Assistant Dean, Professor in the School of Pharmacy Operations department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsGills
Hydrocortisone
Microsomes, Liver
Oncorhynchus mykiss
Oxygenases
Sodium Chloride
Urea
Water
Water-Electrolyte Balance
