Leishmania major chromosome 3 contains two long convergent polycistronic gene clusters separated by a tRNA gene. Nucleic Acids Res 2003 Jul 15;31(14):4201-10
Date
07/11/2003Pubmed ID
12853638Pubmed Central ID
PMC167632DOI
10.1093/nar/gkg469Scopus ID
2-s2.0-0242349118 (requires institutional sign-in at Scopus site) 65 CitationsAbstract
Leishmania parasites (order Kinetoplastida, family Trypanosomatidae) cause a spectrum of human diseases ranging from asymptomatic to lethal. The approximately 33.6 Mb genome is distributed among 36 chromosome pairs that range in size from approximately 0.3 to 2.8 Mb. The complete nucleotide sequence of Leishmania major Friedlin chromosome 1 revealed 79 protein-coding genes organized into two divergent polycistronic gene clusters with the mRNAs transcribed towards the telomeres. We report here the complete nucleotide sequence of chromosome 3 (384 518 bp) and an analysis revealing 95 putative protein-coding ORFs. The ORFs are primarily organized into two large convergent polycistronic gene clusters (i.e. transcribed from the telomeres). In addition, a single gene at the left end is transcribed divergently towards the telomere, and a tRNA gene separates the two convergent gene clusters. Numerous genes have been identified, including those for metabolic enzymes, kinases, transporters, ribosomal proteins, spliceosome components, helicases, an RNA-binding protein and a DNA primase subunit.
Author List
Worthey EA, Martinez-Calvillo S, Schnaufer A, Aggarwal G, Cawthra J, Fazelinia G, Fong C, Fu G, Hassebrock M, Hixson G, Ivens AC, Kiser P, Marsolini F, Rickel E, Salavati R, Sisk E, Sunkin SM, Stuart KD, Myler PJMESH terms used to index this publication - Major topics in bold
AnimalsChromosomes
DNA, Protozoan
Genes, Protozoan
Genome, Protozoan
Leishmania major
Molecular Sequence Data
Multigene Family
RNA, Transfer
Sequence Analysis, DNA
