Faculty Collaboration Database

Response of cardiac mast cells to atrial natriuretic peptide. Am J Physiol Heart Circ Physiol 2007 Aug;293(2):H1216-22

Date

04/17/2007

Pubmed ID

17434981

DOI

10.1152/ajpheart.01388.2006

Scopus ID

2-s2.0-34547920719 (requires institutional sign-in at Scopus site)   13 Citations

Abstract

Previously, our laboratory demonstrated that cardiac mast cell degranulation induces adverse ventricular remodeling in response to chronic volume overload. The purpose of this study was to investigate whether atrial natriuretic peptide (ANP), which is known to be elevated in chronic volume overload, causes cardiac mast cell degranulation. Relative to control, ANP induced significant histamine release from peritoneal mast cells, whereas isolated cardiac mast cells were not responsive. Infusion of ANP (225 pg/ml) into blood-perfused isolated rat hearts produced minimal activation of cardiac mast cells, similar to that seen in the control group. ANP also did not increase matrix metalloproteinase-2 activity, reduce collagen volume fraction, or alter diastolic or systolic cardiac function compared with saline-treated controls. In a subsequent study to evaluate the effects of natriuretic peptide receptor antagonism on volume overload-induced ventricular remodeling, anantin was administered to rats with an aortocaval fistula. Comparable increases of myocardial MMP-2 activity in treated and untreated rats with an aortocaval fistula were associated with equivalent decreases in ventricular collagen (P < 0.05 vs. sham-operated controls). Cardiac functional parameters and left ventricular hypertrophy were unaffected by anantin. We conclude that ANP is not a cardiac mast cell secretagogue and is not responsible for the cardiac mast cell-mediated adverse ventricular remodeling in response to volume overload.

Author List

Murray DB, Gardner JD, Levick SP, Brower GL, Morgan LG, Janicki JS

MESH terms used to index this publication - Major topics in bold

Animals
Aorta, Abdominal
Arteriovenous Fistula
Ascitic Fluid
Atrial Natriuretic Factor
Cell Degranulation
Collagen
Disease Models, Animal
Histamine Release
Hypertrophy, Left Ventricular
In Vitro Techniques
Male
Mast Cells
Matrix Metalloproteinase 2
Myocardium
Peptides, Cyclic
Rats
Rats, Sprague-Dawley
Vena Cava, Inferior
Ventricular Function, Left
Ventricular Remodeling