Brain tumor invasion rate measured in vitro does not correlate with Ki-67 expression. J Neurooncol 1999;45(2):111-6
Date
04/25/2000Pubmed ID
10778726DOI
10.1023/a:1006375316331Scopus ID
2-s2.0-0033499387 (requires institutional sign-in at Scopus site) 24 CitationsAbstract
The need for more accurate prediction of the biological behavior of brain tumors has lead to the use of immunohistochemical methods for assessment of proliferating cell nuclear antigens such as Ki-67. There is a variable association of glioma Ki-67 labeling index with patient survival. Brain invasion by individual tumor cells also defines biological aggressiveness, and can be assessed in vitro. Further, proliferation and migration seem to be mutually exclusive behaviors for a given cell at a point in time. We studied the relationship between Ki-67 labeling index and invasion rate in a group of 10 gliomas, and 2 meningiomas. Human tumor spheroids obtained from operative specimen were co-cultured with fetal rat brain aggregates, and invasion rate was measured by confocal microscopic observation. There was no correlation between two measures of invasion and Ki-67 labeling. This finding supports the dichotomous nature of glioma proliferation and invasion, and may in part explain the limited usefulness of proliferation marker labeling.
Author List
Khoshyomn S, Lew S, DeMattia J, Singer EB, Penar PLAuthor
Sean Lew MD Chief, Professor in the Neurosurgery department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsAstrocytoma
Brain
Brain Neoplasms
Coculture Techniques
Fetus
Glioblastoma
Humans
Ki-67 Antigen
Meningeal Neoplasms
Meningioma
Neoplasm Invasiveness
Neuroectodermal Tumors, Primitive
Oligodendroglioma
Prognosis
Rats
Rats, Sprague-Dawley
Tumor Cells, Cultured
