Faculty Collaboration Database

Characterization of a monoclonal antibody reactive with a glycolipid antigen expressed by tumorigenic and certain immortalized, non-tumorigenic rat esophageal epithelial cell lines. Cancer Immunol Immunother 1993;36(2):94-100

Date

01/01/1993

Pubmed ID

8425214

Pubmed Central ID

PMC11038716

DOI

10.1007/BF01754408

Scopus ID

2-s2.0-0027529807 (requires institutional sign-in at Scopus site)   2 Citations

Abstract

A monoclonal antibody (mAb 5G) was produced against a tumorigenic rat esophageal epithelial cell line, designated B2T. Using an enzyme-linked immunosorbent assay, immunofluorescence assay (IFA), thin-layer chromatography (TLC) and immunoperoxidase staining, it was found that mAb 5G reacted specifically with a glycolipid antigen expressed by three tumorigenic rat esophageal epithelial cell lines, and two out of the three non-tumorigenic, immortalized rat esophageal epithelial cell lines tested; but did not react with primary cultures of normal rat esophageal epithelial cells or fibroblasts. mAb 5G did not bind to rat respiratory tract carcinoma cell lines, to immortalized rat tracheal epithelial cell lines, or to primary cultures of normal rat tracheal epithelial cells. In addition, mAb 5G did not react with any of the human or mouse cell lines tested. In IFA experiments, mAb 5G stained imprints prepared from in vivo propagated B2T tumor tissues, but did not react with normal rat esophageal, tracheal, lung, liver, and kidney tissues. The antigen was identified by TLC as a neutral glycolipid, consisting of two bands, with RF = 0.45 and 0.41, which migrated in proximity to the ceramide trihexoside standard on TLC plates. Densitometric scanning of the antigen bands indicated that the tumorigenic rat esophageal cell lines possessed 50%-90% more mAb-5G-reactive antigen than the non-tumorigenic esophageal cell lines. The results show that mAb 5G reacts specifically with a glycolipid antigen expressed by tumorigenic and certain non-tumorigenic, immortalized rat esophageal epithelial cell lines that might be at the late stages of transformation and early malignancy.

Author List

Wan X, Jamasbi RJ, Stoner GD

MESH terms used to index this publication - Major topics in bold

Animals
Antibodies, Monoclonal
Antigens, Neoplasm
Carcinoma
Cell Line
Enzyme-Linked Immunosorbent Assay
Epithelium
Esophageal Neoplasms
Esophagus
Fibroblasts
Fluorescent Antibody Technique
Glycolipids
Humans
Mice
Mice, Inbred BALB C
Rats
Rats, Inbred F344
Trachea