Conversion of premalignant human cells to tumorigenic cells by methylmethane sulfonate and methylnitronitrosoguanidine. Cell Biol Toxicol 1992;8(4):193-205
Date
10/01/1992Pubmed ID
1337307DOI
10.1007/BF00156730Scopus ID
2-s2.0-0027059254 (requires institutional sign-in at Scopus site) 16 CitationsAbstract
Nine human tumor cell lines derived from both epithelial and mesenchymal tumors exhibited either an anchorage-independent growth non-tumorigenic phenotype or an anchorage-independent tumorigenic phenotype. Transformed epithelial cell lines with the non-tumorigenic phenotype could be converted to a progressively growing tumor phenotype following treatment with either methylmethane sulfonate (MMS) or N-methyl-N'-nitro-N-nitro-soguanidine (MNNG). In contrast, sarcoma derived cell lines with a non-tumorigenic phenotype could be converted to a progressively growing tumor phenotype only with MNNG. SV40 immortalized HET-1A non-tumorigenic phenotype cells could be converted to a progressively growing tumorigenic phenotype, infrequently, when treated with MNNG, but not MMS. Progressively growing tumors produced by either MMS or MNNG treated non-tumorigenic phenotypes exhibited metastatic potential in nude mice. Chemically treated HET-1A cells acquired the ability to produce tumor in mice but the tumor did not exhibit metastatic potential. In contrast, populations of tumorigenic cells were not rendered more biologically aggressive after treatment with either MMS or MNNG; i.e., the latency period for tumor development was not accelerated and the tumors did not exhibit metastatic potential. These results suggest that the biological effects of MMS and MNNG on non-tumorigenic, tumorigenic and immortalized cell lines are phenotype specific.
Author List
Milo GE, Shuler CF, Stoner G, Chen JCMESH terms used to index this publication - Major topics in bold
AnimalsCarcinoma, Small Cell
Carcinoma, Squamous Cell
Cell Line, Transformed
Cell Transformation, Neoplastic
Chondrosarcoma
Fibrosarcoma
Humans
Male
Methyl Methanesulfonate
Methylnitronitrosoguanidine
Mice
Mice, Nude
Osteosarcoma
Phenotype
Rhabdomyosarcoma
Tumor Cells, Cultured
