Faculty Collaboration Database

Inhibition of N-nitrosobenzylmethylamine metabolism and DNA binding in cultured rat esophagus by ellagic acid. Carcinogenesis 1988 Jul;9(7):1313-6

Date

07/01/1988

Pubmed ID

3383347

DOI

10.1093/carcin/9.7.1313

Scopus ID

2-s2.0-0023899029 (requires institutional sign-in at Scopus site)   58 Citations

Abstract

The effect of ellagic acid (EA), a naturally occurring plant phenol, on the metabolism, DNA binding and DNA adduct formation of N-nitrosobenzylmethylamine (NBMA) in cultured explants of rat esophagus was investigated. Explants were incubated in medium containing EA at non-toxic concentrations of 10, 50 and 100 microM for 16 h, followed by the addition of 1 microM [3H]NBMA and EA for 12 h. Explant DNA was isolated by phenol extraction and hydroxylapatite chromatography, and benzaldehyde formation was determined by HPLC analysis of the culture medium. EA produced a significant inhibition in the total covalent binding of NBMA metabolites to DNA and in the production of benzaldehyde in the medium. After acid hydrolysis of the isolated DNA, the NBMA--DNA adducts were separated by HPLC. EA caused a dose-dependent decrease in the formation of N7-methylguanine and O6-methylguanine adducts. These results suggest that EA inhibits both the metabolism of NBMA and the binding of NBMA metabolites to DNA in cultured rat esophagus.

Author List

Mandal S, Shivapurkar NM, Galati AJ, Stoner GD

MESH terms used to index this publication - Major topics in bold

Animals
Benzopyrans
Chromatography, High Pressure Liquid
DNA
DNA Damage
Dimethylnitrosamine
Ellagic Acid
In Vitro Techniques
Rats