Strain-specific susceptibility for pulmonary metastasis of sarcoma 180 cells in inbred mice. Cancer Res 2010 Jun 15;70(12):4859-67
Date
05/27/2010Pubmed ID
20501835Pubmed Central ID
PMC4690845DOI
10.1158/0008-5472.CAN-09-4177Scopus ID
2-s2.0-77953758085 (requires institutional sign-in at Scopus site) 9 CitationsAbstract
Most cancer deaths are a result of metastasis. To extend our understanding of the factors that influence the process, we aimed to develop a mouse model of pulmonary metastasis that can be assayed in multiple inbred mouse strains for further use in identification of host genetic variants that influence metastasis. We used i.v. injection of Sarcoma 180 (S180) cells, which can be tracked and quantified by bioluminescence imaging. We observed growth of S180 cells solely in the lung and observed a wide range of pulmonary metastasis among inbred mouse strains. Interestingly, we noted that the BTBRT+tf/J strain exhibited complete clearance and provide evidence that the mechanism of resistance may involve immune factors, as strains subjected to whole-body irradiation are significantly more susceptible to tumor growth. One possible mechanism of resistance to pulmonary metastasis in BTBRT+tf/J mice may require T-cell function. Our experiments present a new mouse model for further characterization of the genetics and mechanisms of pulmonary metastasis.
Author List
Vikis HG, Jackson EN, Krupnick AS, Franklin A, Gelman AE, Chen Q, Piwnica-Worms D, You MMESH terms used to index this publication - Major topics in bold
AnimalsDisease Models, Animal
Disease Susceptibility
Female
Flow Cytometry
Lung Neoplasms
Lymphocyte Depletion
Mice
Mice, Inbred A
Mice, Inbred AKR
Mice, Inbred C57BL
Mice, Inbred DBA
Neoplasm Transplantation
Sarcoma 180
Species Specificity
T-Lymphocytes
Whole-Body Irradiation
