Neonatal gene transfer of Serca2a delays onset of hypertrophic remodeling and improves function in familial hypertrophic cardiomyopathy. J Mol Cell Cardiol 2010 Dec;49(6):993-1002
Date
09/22/2010Pubmed ID
20854827Pubmed Central ID
PMC2982190DOI
10.1016/j.yjmcc.2010.09.010Scopus ID
2-s2.0-78149412295 (requires institutional sign-in at Scopus site) 46 CitationsAbstract
Familial hypertrophic cardiomyopathy (FHC) is an autosomal dominant genetic disorder linked to numerous mutations in the sarcomeric proteins. The clinical presentation of FHC is highly variable, but it is a major cause of sudden cardiac death in young adults with no specific treatments. We tested the hypothesis that early intervention in Ca(2+) regulation may prevent pathological hypertrophy and improve cardiac function in a FHC displaying increased myofilament sensitivity to Ca(2+) and diastolic dysfunction. A transgenic (TG) mouse model of FHC with a mutation in tropomyosin at position 180 was employed. Adenoviral-Serca2a (Ad.Ser) was injected into the left ventricle of 1-day-old non-transgenic (NTG) and TG mice. Ad.LacZ was injected as a control. Serca2a protein expression was significantly increased in NTG and TG hearts injected with Ad.Ser for up to 6 weeks. Compared to TG-Ad.LacZ hearts, the TG-Ad.Ser hearts showed improved whole heart morphology. Moreover, there was a significant decline in ANF and β-MHC expression. Developed force in isolated papillary muscle from 2- to 3-week-old TG-Ad.Ser hearts was higher and the response to isoproterenol (ISO) improved compared to TG-Ad.LacZ muscles. In situ hemodynamic measurements showed that by 3 months the TG-Ad.Ser hearts also had a significantly improved response to ISO compared to TG-Ad.LacZ hearts. The present study strongly suggests that Serca2a expression should be considered as a potential target for gene therapy in FHC. Moreover, our data imply that development of FHC can be successfully delayed if therapies are started shortly after birth.
Author List
Peña JR, Szkudlarek AC, Warren CM, Heinrich LS, Gaffin RD, Jagatheesan G, del Monte F, Hajjar RJ, Goldspink PH, Solaro RJ, Wieczorek DF, Wolska BMMESH terms used to index this publication - Major topics in bold
Actin CytoskeletonAdenoviridae
Animals
Animals, Newborn
Atrial Natriuretic Factor
Calcium-Binding Proteins
Cardiomyopathy, Hypertrophic, Familial
Gene Transfer Techniques
Genetic Therapy
Heart Function Tests
Hemodynamics
Humans
Injections
Isoproterenol
Mice
Mice, Transgenic
Myocardial Contraction
Myosin Heavy Chains
Phosphorylation
Protein Isoforms
Rabbits
Sarcoplasmic Reticulum Calcium-Transporting ATPases
Ventricular Remodeling
