The vaccinia virus A4OR gene product is a nonstructural, type II membrane glycoprotein that is expressed at the cell surface. J Gen Virol 1999 Aug;80 ( Pt 8):2137-2148
Date
08/31/1999Pubmed ID
10466813DOI
10.1099/0022-1317-80-8-2137Scopus ID
2-s2.0-0032771586 (requires institutional sign-in at Scopus site) 38 CitationsAbstract
Gene A40R from vaccinia virus (VV) strain Western Reserve has been characterized. The open reading frame (ORF) was predicted to encode a 159 amino acid, 18152 Da protein with amino acid similarity to C-type animal lectins and to the VV A34R protein, a component of extracellular enveloped virus (EEV). Northern blotting and S1 nuclease mapping showed that gene A40R is transcribed early during infection from a position 12 nucleotides upstream of the ORF, producing a transcript of approximately 600 nucleotides. Rabbit anti-sera were raised against bacterial fusion proteins containing parts of the A40R protein. These were used to identify an 18 kDa primary translation product and N- and O-glycosylated forms of 28, 35 and 38 kDa. The A40R proteins were detected early during infection, formed higher molecular mass complexes under non-reducing conditions and were present on the cell surface but absent from virions. The proteins partitioned with integral membrane proteins in Triton X-114. Canine pancreatic microsomal membranes protected in vitro-translated A40R from proteinase K digestion, suggesting the A40R protein has type II membrane topology. A mutant virus with the A40R gene disrupted after amino acid 50, so as to remove the entire lectin-like domain, and a revertant virus were constructed. Disruption of the A40R gene did not affect virus plaque size, in vitro growth rate and titre, EEV formation, or virus virulence in a murine intranasal model.
Author List
Wilcock D, Duncan SA, Traktman P, Zhang WH, Smith GLMESH terms used to index this publication - Major topics in bold
Administration, IntranasalAnimals
Cell Extracts
Cell Line
Cell Membrane
Dogs
Female
Genome, Viral
Glycoproteins
HeLa Cells
Humans
Membrane Glycoproteins
Mice
Mice, Inbred BALB C
Octoxynol
Polyethylene Glycols
Protein Biosynthesis
Rabbits
Sequence Analysis
Transcription, Genetic
Vaccinia virus
Vero Cells
Viral Nonstructural Proteins
Virion
