Faculty Collaboration Database

Interactive gene expression pattern in prostate cancer cells exposed to phenolic antioxidants. Life Sci 2002 Mar 01;70(15):1821-39

Date

05/11/2002

Pubmed ID

12002526

DOI

10.1016/s0024-3205(02)01481-9

Scopus ID

2-s2.0-0036498948 (requires institutional sign-in at Scopus site)   61 Citations

Abstract

Dietary phenolic compounds are known to elicite vital cellular responses such as cell cycle arrest, apoptosis and differentiation by activating a cascade of molecular events. As there is an increasing interest to improve the efficacy of these compounds for use as potential chemopreventive agents, we wanted to understand the impact of phenolic compounds on target genes in prostate cancer. In this study we used human cDNA microarrays with 2400 clones consisting of 17 prosite motifs to characterize alterations in gene expression pattern in response to the phenolic antioxidants ellagic acid (EA) and resveratrol (RE). Over a 48-hr exposure of androgen - sensitive LNCaP cells to EA and RE, a total of 593 and 555 genes respectively, showed more than a two fold difference in expression. A distinct set of genes in both EA-and RE-treated cells may represent the signature profile of phenolic antioxidant-induced gene expression in LNCaP cells. Although extensive similarity was found between effects of EA - and RE - responsive genes in prostate cancer cells, out of 246 genes with overlapping responses, 25 genes showed an opposite effect. Quantitative RT-PCR was used to verify and validate the differential expression of selected genes identified from cDNA microarrays. In-depth analysis of the data from this study provided insight into the alterations in the p53 - responsive genes, p300, Apaf-1, NF-kBp50 and p65 and PPAR families of genes, suggesting the activation of multiple signaling pathways that leads to growth inhibition of LNCaP cells. This is a first study to look for changes in a large number of human genes in response to dietary compounds.

Author List

Narayanan BA, Narayanan NK, Stoner GD, Bullock BP

MESH terms used to index this publication - Major topics in bold

Anticarcinogenic Agents
Antioxidants
Carcinoma
DNA, Neoplasm
Ellagic Acid
Gene Expression Profiling
Gene Expression Regulation, Neoplastic
Humans
Male
Oligonucleotide Array Sequence Analysis
Phenols
Prostatic Neoplasms
RNA, Messenger
Reverse Transcriptase Polymerase Chain Reaction
Stilbenes
Tumor Cells, Cultured