Suppression of N-nitrosomethylbenzylamine-induced rat esophageal tumorigenesis by dietary feeding of auraptene. J Exp Clin Cancer Res 2000 Mar;19(1):45-52
Date
06/07/2000Pubmed ID
10840935Scopus ID
2-s2.0-0034075996 (requires institutional sign-in at Scopus site) 31 CitationsAbstract
The modifying effects of auraptene on N-nitrosomethylbenzylamine (NMBA)-induced esophageal tumorigenesis were investigated in male F344 rats. At 5 weeks of age, all animals, except those with the test chemical alone and control rats, received s.c. injections of NMBA (0.5 mg/kg body weight/injection, three times per week) for 5 weeks. At the end of the study (20 weeks), 75% of the rats treated with NMBA alone had esophageal neoplasms (papillomas). However, the groups who received a dose of 500 ppm auraptene during the initiation phase developed significantly reduced incidence of tumors (39%; P<0.05). Exposure to auraptene (500 ppm) during the post-initiation phase also decreased the frequency of the tumors (29%; P<0.01). The reduction of the incidence of severe dysplasia was obtained when auraptene was administered in the post-initiation phase (P<0.05). Cell proliferation in the esophageal epithelium determined by proliferating cell nuclear antigen (PCNA) was lowered by auraptene (P<0.01). Blood polyamine contents in rats who received NMBA and the test compound were also smaller than those of rats that received the carcinogen (P<0.05). These findings suggest that dietary auraptene is effective in inhibiting the development of esophageal tumors by NMBA when given during the initiation as well as post-initiation phases, and such inhibition is related to suppression of cell proliferation in the esophageal epithelium.
Author List
Kawabata K, Tanaka T, Yamamoto T, Hara A, Murakami A, Koshimizu K, Ohigashi H, Stoner GD, Mori HMESH terms used to index this publication - Major topics in bold
AnimalsAnticarcinogenic Agents
Carcinogens
Coumarins
Diet
Dimethylnitrosamine
Esophageal Neoplasms
Incidence
Male
Rats
Rats, Inbred F344
