Genetic evidence for involvement of vaccinia virus DNA-dependent ATPase I in intermediate and late gene expression. J Virol 1989 Sep;63(9):3999-4010
Date
09/01/1989Pubmed ID
2527312Pubmed Central ID
PMC250997DOI
10.1128/JVI.63.9.3999-4010.1989Scopus ID
2-s2.0-0024312640 (requires institutional sign-in at Scopus site) 16 CitationsAbstract
To delineate the role of the vaccinia virus-encapsidated DNA-dependent ATPase I in the life cycle of the virus, we performed a detailed study of two temperature-sensitive mutants with lesions in the gene encoding the enzyme. Profiles of viral DNA and protein accumulation during infection showed the mutants to be competent for DNA synthesis but deficient in late protein synthesis, confirming their defective late phenotype (R. C. Condit and A. Motyczka, Virology 113:224-241, 1981: R. C. Condit, A. Motyczka, and G. Spizz, Virology 128:429-443, 1983). In vitro translation of viral RNA and S1 nuclease mapping of selected mRNAs demonstrated that the deficit in late protein synthesis stemmed from a defect in the transcriptional machinery. Intermediate and late gene expression appeared to be most affected. The transcriptional defect was of unequal severity in the two mutants. However, their phenotypes were indistinguishable and their respective lesions were mapped to the same 300 nucleotides at the 5' end of the gene. DNA sequence analysis assigned a single nucleotide and amino acid change to one of the mutants.
Author List
Künzi MS, Traktman PMESH terms used to index this publication - Major topics in bold
Adenosine TriphosphatasesBase Sequence
DNA Helicases
DNA Replication
DNA, Viral
Genes, Viral
Molecular Sequence Data
Mutation
Protein Biosynthesis
RNA, Messenger
Transcription, Genetic
Vaccinia virus
Viral Proteins
Virus Replication
