Molecular genetic analysis of vaccinia virus DNA polymerase mutants. J Virol 1989 Feb;63(2):841-6
Date
02/01/1989Pubmed ID
2911123Pubmed Central ID
PMC247758DOI
10.1128/JVI.63.2.841-846.1989Scopus ID
2-s2.0-0024504467 (requires institutional sign-in at Scopus site) 36 CitationsAbstract
To further our understanding of the structure and function of the vaccinia virus DNA polymerase, we have performed fine genetic analysis of three mutants with lesions in the polymerase gene. By performing marker rescue analysis with DNA fragments of decreasing size, each lesion was localized to within 500 base pairs of DNA. The relevant regions of the mutant alleles were then cloned and subjected to DNA sequence analysis, which allowed the assignment of a single nucleotide and amino acid change to each mutant. As well as providing structure-function correlations germane to an understanding of polymerase activity, these data have provided insights into the frequency and possible mechanisms of viral homologous recombination.
Author List
Traktman P, Kelvin M, Pacheco SAuthor
Paula Traktman Duncan PhD Emeritus Professor in the Microbiology and Immunology department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Amino Acid SequenceBase Sequence
DNA, Viral
DNA-Directed DNA Polymerase
Genes
Genes, Viral
Molecular Sequence Data
Recombination, Genetic
Vaccinia virus
Viral Proteins
