Decreased TRH receptor mRNA activity precedes homologous downregulation: assay in oocytes. Science 1987 Dec 04;238(4832):1406-8
Date
12/04/1987Pubmed ID
2825350DOI
10.1126/science.2825350Scopus ID
2-s2.0-0023574359 (requires institutional sign-in at Scopus site) 59 CitationsAbstract
Ligand-induced decrease in cell-surface receptor number (homologous downregulation) is often due to rapid receptor internalization. Thyrotropin-releasing hormone (TRH), however, causes a slow downregulation of TRH receptors (TRH-Rs), with a half-time of approximately 12 hours, in GH3 rat pituitary cells. The mechanism of TRH-R downregulation was studied by monitoring TRH-evoked depolarizing currents in Xenopus oocytes injected with GH3 cell RNA as a bioassay for TRH-R messenger RNA (mRNA) activity. In GH3 cells, TRH caused a rapid decrease in TRH-R mRNA activity to 15 percent of control within 3 hours. Because the half-life of TRH-R mRNA activity in control cells was approximately 3 hours, the rapid decrease in mRNA activity was not due to inhibition of mRNA synthesis alone and may represent a post-transcriptional effect.
Author List
Oron Y, Straub RE, Traktman P, Gershengorn MCAuthor
Paula Traktman Duncan PhD Emeritus Professor in the Microbiology and Immunology department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsFemale
Gene Expression Regulation
Membrane Potentials
Neoplasm Proteins
Oocytes
Pituitary Neoplasms
RNA, Messenger
RNA, Neoplasm
Rats
Receptors, Neurotransmitter
Receptors, Thyrotropin-Releasing Hormone
Thyrotropin-Releasing Hormone
Tumor Cells, Cultured
Xenopus laevis
