Isolation and properties of Moloney murine leukemia virus mutants: use of a rapid assay for release of virion reverse transcriptase. J Virol 1981 Apr;38(1):239-48
Date
04/01/1981Pubmed ID
6165830Pubmed Central ID
PMC171145DOI
10.1128/JVI.38.1.239-248.1981Scopus ID
2-s2.0-0019507492 (requires institutional sign-in at Scopus site) 371 CitationsAbstract
A rapid assay for retroviral reverse transcriptase activity released into the culture medium by infected cells was developed. With the assay, 4,000 clonally infected cell lines could be tested in a few hours. We have adapted the assay for use as a screen for the detection of spontaneous viral mutants. Mutants of Moloney murine leukemia virus have been isolated which (i) produce a thermolabile reverse transcriptase, (ii) are temperature sensitive for release of enzyme activity, or (iii) can only productively infect cells already producing gag-related polypeptides. The assay has also been useful for the isolation of nonproducer cells infected with various replication-defective transforming viruses.
Author List
Goff S, Traktman P, Baltimore DAuthor
Paula Traktman Duncan PhD Emeritus Professor in the Microbiology and Immunology department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Culture MediaMolecular Weight
Moloney murine leukemia virus
Mutation
RNA-Directed DNA Polymerase
Temperature
Viral Plaque Assay
