Temperature-sensitive mutants with lesions in the vaccinia virus F10 kinase undergo arrest at the earliest stage of virion morphogenesis. J Virol 1995 Oct;69(10):6581-7
Date
10/01/1995Pubmed ID
7666563Pubmed Central ID
PMC189564DOI
10.1128/JVI.69.10.6581-6587.1995Scopus ID
2-s2.0-0029130581 (requires institutional sign-in at Scopus site) 84 CitationsAbstract
Vaccinia virus encodes two protein kinases; the B1 kinase is expressed early and appears to play a role during DNA replication, whereas the F10 kinase is expressed late and is encapsidated in virions. Here we report that the F10 kinase gene is the locus affected in a complementation group of temperature-sensitive mutants composed of ts15, ts28, ts54, and ts61. Although these mutants have a biochemically normal phenotype at the nonpermissive temperature, directing the full program of viral gene expression, they fail to form mature virions. Electron microscopic analysis indicates that morphogenesis undergoes arrest at a very early stage, prior to the formation of membrane crescents or immature virions. An essential role for the F10 protein kinase in orchestrating the onset of virion assembly is implied.
Author List
Traktman P, Caligiuri A, Jesty SA, Liu K, Sankar UMESH terms used to index this publication - Major topics in bold
AnimalsCell Line
Gene Expression
Genes, Viral
Genetic Complementation Test
Microscopy, Electron
Morphogenesis
Mutation
Open Reading Frames
Phenotype
Polymerase Chain Reaction
Restriction Mapping
Temperature
Vaccinia virus
Viral Proteins
