Faculty Collaboration Database

Uptake and presentation of antigen to T cells by primary colonic epithelial cells in normal and diseased states. Gastroenterology 2000 Dec;119(6):1548-59

Date

12/13/2000

Pubmed ID

11113076

DOI

10.1053/gast.2000.20168

Scopus ID

2-s2.0-0034466148 (requires institutional sign-in at Scopus site)   34 Citations

Abstract

BACKGROUND & AIMS: The immunoregulatory properties of primary colonic epithelial cells (CECs) have not been defined. The ability of CECs from wild-type and interleukin 2-deficient (IL-2(-/-)) mice to take up a complex protein antigen and present peptides via MHC molecules to T cells was assessed and contrasted with that of primary small intestinal epithelial cells (SIECs).

METHODS: Uptake of fluorescein isothiocyanate (FITC)-labeled ovalbumin (FITC-OVA) by CECs and SIECs from wild-type and IL-2(-/-) mice was measured by flow cytometry. The effect of disrupting cytoskeleton organization and metabolic activity of CEC on antigen uptake was assessed. An OVA/I-A(b)-specific CD4(+) T-cell line transfected with an NFAT-lacZ reporter gene construct was used to evaluate the ability of CECs and SIECs as well as CECs from healthy and colitic IL-2(-/-) mice to present antigen to T cells.

RESULTS: Uptake of FITC-OVA by CECs is concentration dependent, is not saturated at physiologic concentrations, and requires metabolically active cells. CECs from IL-2(-/-) mice take up significantly more antigen than those from wild-type mice. CECs are more efficient APCs than SIECs, and antigen-pulsed CECs from IL-2(-/-) mice induce the highest levels of T-cell activation.

CONCLUSIONS: Primary CECs are efficient APCs for CD4 MHC class II-restricted T cells. Antigen uptake and presentation is up-regulated in animals prone to develop intestinal inflammation.

Author List

Telega GW, Baumgart DC, Carding SR

MESH terms used to index this publication - Major topics in bold

Animals
Antigen-Presenting Cells
Antigens
CD4-Positive T-Lymphocytes
Cell Line
Colitis
Colon
Enteritis
Female
Fluorescein-5-isothiocyanate
Fluorescent Dyes
Interleukin-2
Intestinal Mucosa
Intestine, Small
Mice
Mice, Inbred C57BL
Ovalbumin
Reference Values