Faculty Collaboration Database

Pluripotent stem cell heterogeneity and the evolving role of proteomic technologies in stem cell biology. Proteomics 2011 Oct;11(20):3947-61

Date

08/13/2011

Pubmed ID

21834136

Pubmed Central ID

PMC3311164

DOI

10.1002/pmic.201100100

Scopus ID

2-s2.0-80054006441 (requires institutional sign-in at Scopus site)   20 Citations

Abstract

Stem cells represent obvious choices for regenerative medicine and are invaluable for studies of human development and drug testing. The proteomic landscape of pluripotent stem cells (PSCs), in particular, is not yet clearly defined; consequently, this field of research would greatly benefit from concerted efforts designed to better characterize these cells. In this concise review, we provide an overview of stem cell potency, highlight the types and practical implications of heterogeneity in PSCs and provide a detailed analysis of the current view of the pluripotent proteome in a unique resource for this rapidly evolving field. Our goal in this review is to provide specific insights into the current status of the known proteome of both mouse and human PSCs. This has been accomplished by integrating published data into a unified PSC proteome to facilitate the identification of proteins, which may be informative for the stem cell state as well as to reveal areas where our current view is limited. These analyses provide insight into the challenges faced in the proteomic analysis of PSCs and reveal one area--the cell surface subproteome--that would especially benefit from enhanced research efforts.

Author List

Gundry RL, Burridge PW, Boheler KR

MESH terms used to index this publication - Major topics in bold

Animals
Cell Differentiation
Humans
Mice
Pluripotent Stem Cells
Proteomics