Faculty Collaboration Database

Pulmonary reduction of an intravascular redox polymer. Am J Physiol Lung Cell Mol Physiol 2001 Jun;280(6):L1290-9

Date

05/15/2001

Pubmed ID

11350810

DOI

10.1152/ajplung.2001.280.6.L1290

Scopus ID

2-s2.0-0034983836 (requires institutional sign-in at Scopus site)   17 Citations

Abstract

Pulmonary endothelial cells in culture reduce external electron acceptors via transplasma membrane electron transport (TPMET). In studying endothelial TPMET in intact lungs, it is difficult to exclude intracellular reduction and reducing agents released by the lung. Therefore, we evaluated the role of endothelial TPMET in the reduction of a cell-impermeant redox polymer, toluidine blue O polyacrylamide (TBOP(+)), in intact rat lungs. When added to the perfusate recirculating through the lungs, the venous effluent TBOP(+) concentration decreased to an equilibrium level reflecting TBOP(+) reduction and autooxidation of its reduced (TBOPH) form. Adding superoxide dismutase (SOD) to the perfusate increased the equilibrium TBOP(+) concentration. Kinetic analysis indicated that the SOD effect could be attributed to elimination of the superoxide product of TBOPH autooxidation rather than of superoxide released by the lungs, and experiments with lung-conditioned perfusate excluded release of other TBOP(+) reductants in sufficient quantities to cause significant TBOP(+) reduction. Thus the results indicate that TBOP(+) reduction is via TPMET and support the utility of TBOP(+) and the kinetic model for investigating TPMET mechanisms and their adaptations to physiological and pathophysiological stresses in the intact lung.

Author List

Audi SH, Bongard RD, Okamoto Y, Merker MP, Roerig DL, Dawson CA

Author

Said Audi PhD Professor in the Biomedical Engineering department at Marquette University

MESH terms used to index this publication - Major topics in bold

Acrylic Resins
Animals
Ascorbic Acid
Blood Flow Velocity
Cytochrome c Group
In Vitro Techniques
Kinetics
Lung
Models, Biological
Oxidation-Reduction
Perfusion
Pulmonary Circulation
Rats
Spectrophotometry
Superoxide Dismutase
Superoxides
Tolonium Chloride