Ancestral bias in the Hras1 gene and distal Chromosome 7 among inbred mice. Mamm Genome 2007 Oct;18(10):732-8
Date
10/02/2007Pubmed ID
17906893Pubmed Central ID
PMC2042029DOI
10.1007/s00335-007-9061-1Scopus ID
2-s2.0-36148957104 (requires institutional sign-in at Scopus site)Abstract
Inbred strains of mice vary in their frequency of liver tumors initiated by a mutation in the Hras1 (H-ras) proto-oncogene. We sequenced 4.5 kb of the Hras1 gene on distal Chr 7 in a diverse set of 12 commonly used laboratory inbred strains of mice and detected no sequence variation to account for strain-specific differences in Hras1 mutation prevalence. Furthermore, the Hras1 sequence is essentially monoallelic for an ancestral gene derived from the M. m. domesticus species. To determine if the monoallelism and associated low rate of polymorphism are unique to Hras1 or representative of the general chromosomal locale, we extended the sequence analysis to 12 genes in the final 8 Mb of distal Chr 7. A region of at least 2.5 Mb that encompasses several genes, including Hras1 and the H19/Igf2 loci, demonstrates virtually no sequence variation. The 12 inbred strains share one dominant haplotype derived from the M. m. domesticus allele. Chromosomal regions flanking the monoallelic segment exhibit a significantly higher rate of variation and multiple haplotypes, a majority of which are attributed to M. m. domesticus or M. m. musculus ancestry.
Author List
Drew JC, Kastenmeier AS, Drinkwater NRAuthor
Andrew Sean Kastenmeier MD Associate Professor in the Surgery department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AllelesAnimals
Chromosome Mapping
Chromosomes
Gene Expression Regulation
Haplotypes
Mice
Mice, Inbred Strains
Models, Genetic
Mutation
Phylogeny
Polymorphism, Single Nucleotide
Proto-Oncogene Proteins p21(ras)
Quantitative Trait Loci
