Isolation of the thrombospondin membrane receptor. J Clin Invest 1987 Apr;79(4):1054-61
Date
04/01/1987Pubmed ID
2435757Pubmed Central ID
PMC424283DOI
10.1172/JCI112918Scopus ID
2-s2.0-0023251127 (requires institutional sign-in at Scopus site) 449 CitationsAbstract
Thrombospondin (TSP), a 450-kD multifunctional glycoprotein with a broad tissue distribution, is secreted upon platelet stimulation, binds to the activated platelet surface, and supports platelet aggregation. We have identified and isolated an 88-kd membrane glycoprotein present in platelets, endothelial cells, monocytes, and a variety of human tumor cell lines that is the membrane binding site for TSP. Endogenous platelet TSP binding to thrombin- and ionophore-stimulated human platelets was inhibited in the presence of the monoclonal antibody OKM5. TSP binding to C32 melanoma cells and HT1080 fibrosarcoma cells was specific and also inhibitable with OKM5 Mab. Cell labeling followed by specific immunoprecipitation demonstrated biosynthesis of a single 88-kD glycoprotein. Binding of TSP to the isolated membrane protein was specific and saturable. These studies identify an 88-kD membrane glycoprotein that reacts with the monoclonal antibody, OKM5, and may function as the cellular TSP receptor.
Author List
Asch AS, Barnwell J, Silverstein RL, Nachman RLAuthor
Roy L. Silverstein MD Professor in the Medicine department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Antibodies, MonoclonalBlood Platelets
CD36 Antigens
Cell Line
Fibrosarcoma
Glycoproteins
Humans
Melanoma
Membrane Proteins
Molecular Weight
Receptors, Mitogen
Thrombin
Thrombospondins
