Faculty Collaboration Database

Respiration-linked proton translocation coupled to anaerobic reduction of manganese(IV) and iron(III) in Shewanella putrefaciens MR-1. J Bacteriol 1990 Nov;172(11):6232-8

Date

11/01/1990

Pubmed ID

2172208

Pubmed Central ID

PMC526804

DOI

10.1128/jb.172.11.6232-6238.1990

Scopus ID

2-s2.0-0025018122 (requires institutional sign-in at Scopus site)   251 Citations

Abstract

An oxidant pulse technique, with lactate as the electron donor, was used to study respiration-linked proton translocation in the manganese- and iron-reducing bacterium Shewanella putrefaciens MR-1. Cells grown anaerobically with fumarate or nitrate as the electron acceptor translocated protons in response to manganese (IV), fumarate, or oxygen. Cells grown anaerobically with fumarate also translocated protons in response to iron(III) and thiosulfate, whereas those grown with nitrate did not. Aerobically grown cells translocated protons only in response to oxygen. Proton translocation with all electron acceptors was abolished in the presence of the protonophore carbonyl cyanide m-chlorophenylhydrazone (20 microM) and was partially to completely inhibited by the electron transport inhibitor 2-n-heptyl-4-hydroxyquinoline N-oxide (50 microM).

Author List

Myers CR, Nealson KH

MESH terms used to index this publication - Major topics in bold

Aerobiosis
Anaerobiosis
Carbonyl Cyanide m-Chlorophenyl Hydrazone
Electron Transport
Fumarates
Gram-Negative Anaerobic Bacteria
Iron
Kinetics
Manganese
Oxygen Consumption
Protons