What leads to different mediators of alkalosis-induced vasodilation in isolated and in situ pulmonary vessels? Am J Physiol Lung Cell Mol Physiol 2003 May;284(5):L799-807
Date
01/28/2003Pubmed ID
12547732DOI
10.1152/ajplung.00402.2002Scopus ID
2-s2.0-0037404358 (requires institutional sign-in at Scopus site) 10 CitationsAbstract
We previously found that nitric oxide synthase (NOS) inhibition fully blocked alkalosis-induced relaxation of piglet pulmonary artery and vein rings. In contrast, NOS inhibition alone had no effect on alkalosis-induced pulmonary vasodilation in isolated piglet lungs. This study sought to identify factors contributing to the discordance between isolated and in situ pulmonary vessels. The roles of pressor stimulus (hypoxia vs. the thromboxane mimetic U-46619), perfusate composition (blood vs. physiological salt solution), and flow were assessed. Effects of NOS inhibition on alkalosis-induced dilation were also directly compared in 150-350-microm-diameter cannulated arteries and 150-900-microm-diameter, angiographically visualized, in situ arteries. Finally, effects of NOS inhibition on alkalosis-induced vasodilation were measured in intact piglets. NOS inhibition with N(omega)-nitro-L-arginine fully abolished alkalosis-induced vasodilation in all cannulated arteries but failed to alter alkalosis-induced vasodilation in intact lungs. The results indicate that investigation of other factors, such as perivascular tissue (e.g., adventitia and parenchyma) and remote signaling pathways, will need to be carried out to reconcile this discordance between isolated and in situ arteries.
Author List
Gordon JB, VanderHeyden MA, Halla TR, Cortez EP, Hernandez G, Haworth ST, Dawson CA, Madden JAAuthor
Test W. User test user title in the Anesthesiology department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic AcidAlkalosis
Animals
Animals, Newborn
Blood Flow Velocity
Catheterization
Enzyme Inhibitors
Hypoxia
Nitroarginine
Perfusion
Pulmonary Artery
Pulmonary Circulation
Swine
Vasoconstrictor Agents
Vasodilation
