Faculty Collaboration Database

Use of immobilized platelet membrane glycoproteins for the detection of platelet-specific alloantibodies in solid-phase ELISA. Vox Sang 1987;53(3):157-61

Date

01/01/1987

Pubmed ID

3318122

DOI

10.1111/j.1423-0410.1987.tb04941.x

Scopus ID

2-s2.0-0023271293 (requires institutional sign-in at Scopus site)   12 Citations

Abstract

Platelet membrane glycoproteins were isolated from intact platelets by detergent-phase extraction, fixed to the wells of microtiter trays and used as targets for the detection of platelet-reactive alloantibodies by enzymelinked immunospecific assay (ELISA). The final preparations contained 0.4% of total platelet protein. Antibodies reactive with antigens PlA1, PlA2, Baka, Pena and HLA-A2 were specifically detected at dilutions ranging form 1:640 to 1:1.600. Under the conditions utilized, the ELISA was more sensitive than assays involving 51Cr, radiolabeled monoclonal anti-IgG binding, and indirect immunofluorescence testing by one order of magnitude or greater. When platelets were pretreated with chloroquine to remove class I HLA antigens prior to detergent-phase extraction, reactions with HLA-specific antibodies were lost, but reactions with platelet-specific alloantibodies were retained. This approach offers a simple, sensitive and rapid method to detect and identify platelet-specific alloantibodies in sera containing HLA-reactive alloantibodies.

Author List

Collins J, Aster RH

Author

Richard Aster MD Emeritus Professor in the Medicine department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Antibodies
Blood Platelets
Chloroquine
Enzyme-Linked Immunosorbent Assay
Evaluation Studies as Topic
Fluorescent Antibody Technique
HLA Antigens
Humans
Isoantibodies
Platelet Membrane Glycoproteins