Modeling hepatitis C virus infection using human induced pluripotent stem cells. Proc Natl Acad Sci U S A 2012 Feb 14;109(7):2544-8
Date
02/07/2012Pubmed ID
22308485Pubmed Central ID
PMC3289320DOI
10.1073/pnas.1121400109Scopus ID
2-s2.0-84857125662 (requires institutional sign-in at Scopus site) 196 CitationsAbstract
Human pathogens impact patient health through a complex interplay with the host, but models to study the role of host genetics in this process are limited. Human induced pluripotent stem cells (iPSCs) offer the ability to produce host-specific differentiated cells and thus have the potential to transform the study of infectious disease; however, no iPSC models of infectious disease have been described. Here we report that hepatocyte-like cells derived from iPSCs support the entire life cycle of hepatitis C virus, including inflammatory responses to infection, enabling studies of how host genetics impact viral pathogenesis.
Author List
Schwartz RE, Trehan K, Andrus L, Sheahan TP, Ploss A, Duncan SA, Rice CM, Bhatia SNMESH terms used to index this publication - Major topics in bold
Blotting, WesternCells, Cultured
Fluorescent Antibody Technique
Hepatitis C
Humans
Models, Theoretical
Pluripotent Stem Cells
Reverse Transcriptase Polymerase Chain Reaction
