Bone marrow transplantation for severe aplastic anemia using a phenotypically HLA-identical, SB-compatible unrelated donor. Transplantation 1983 Jun;35(6):566-71
Date
06/01/1983Pubmed ID
6223416DOI
10.1097/00007890-198306000-00010Scopus ID
2-s2.0-0020505364 (requires institutional sign-in at Scopus site) 48 CitationsAbstract
A three-year-old boy with severe aplastic anemia (HLA-A1,B8(Bw6), Cw7,DR3, MB2, MT2, SB4/A1,B8 (Bw6), Cw7,DR3,MB2,MT2,SB-) received a bone marrow transplant from a phenotypically HLA-identical, SB-compatible female unrelated donor. This donor was selected from eighteen HLA-A1,-B8,-blood donors after extended serotyping, mixed leukocyte culture testing and secondary proliferation assays with primed lymphocyte typing reagents specific for SB. Although patient cells proliferated well as responders in MLR, their stimulatory capability was greatly impaired. Because the patient had inherited the same serological HLA-D haplotype from each parent, it was concluded that a compatible unrelated donor must be homozygous for the same HLA-D antigens as the patient. This HLA-D homozygosity was demonstrated by the lack of MLR responses of both parents to stimulators from the donor. The SB typing results suggested SB compatibility because both the patient and the donor typed as SB4,-. Following bone marrow transplantation, there was rapid hematopoietic engraftment. The patient developed severe diarrhea caused by graft-versus-host disease of the gastrointestinal tract, which necessitated hyperalimentation. He is currently eighteen months posttransplant with full hematopoietic reconstitution and moderate chronic skin graft-versus-host disease.
Author List
Duquesnoy RJ, Zeevi A, Marrari M, Hackbarth S, Camitta BMESH terms used to index this publication - Major topics in bold
Anemia, AplasticBone Marrow Transplantation
Child, Preschool
Female
Genes, MHC Class II
Genotype
HLA Antigens
HLA-DR Antigens
Humans
Long-Term Care
Lymphocyte Culture Test, Mixed
Male
Middle Aged
Phenotype
Tissue Donors
