Faculty Collaboration Database

Presynaptic modulation of ganglionic ACh release by muscarinic and nicotinic receptors. Am J Physiol 1990 Aug;259(2 Pt 2):R288-93

Date

08/01/1990

Pubmed ID

2386239

DOI

10.1152/ajpregu.1990.259.2.R288

Scopus ID

2-s2.0-0025046943 (requires institutional sign-in at Scopus site)   14 Citations

Abstract

The present experiments were undertaken to investigate the effects of atropine and d-tubocurarine on acetylcholine (ACh) release and ganglionic synaptic transmission in the isolated cat stellate ganglion. Ganglionic release of picomole amounts of ACh was measured by radioenzymatic assay, and ganglionic transmission was estimated on the basis of the compound action potential recorded from the postganglionic stellate cardiac nerve. Atropine (5 microM) produced a significant increase in both spontaneous and evoked ACh release from the ganglion while depressing synaptic transmission. d-Tubocurarine (20 microM) also caused a significant, though smaller, increase in spontaneous release of ACh but had little effect on evoked release of ACh. These results suggest that ACh release and synaptic transmission in the cat stellate ganglion are subject to cholinergic feedback regulation, which appears to be mediated predominantly via muscarinic presynaptic receptors.

Author List

Dujic Z, Roerig DL, Schedewie HK, Kampine JP, Bosnjak ZJ

MESH terms used to index this publication - Major topics in bold

Acetylcholine
Action Potentials
Animals
Atropine
Cats
Dose-Response Relationship, Drug
Electric Stimulation
Female
Ganglia, Sympathetic
Male
Physostigmine
Receptors, Muscarinic
Receptors, Nicotinic
Synapses
Tubocurarine