Faculty Collaboration Database

An automated coronary artery occlusion device for stimulating collateral development in vivo. J Pharmacol Toxicol Methods 2002;48(2):111-8

Date

10/21/2003

Pubmed ID

14565568

DOI

10.1016/S1056-8719(03)00040-6

Scopus ID

2-s2.0-0142043898 (requires institutional sign-in at Scopus site)   5 Citations

Abstract

INTRODUCTION: Repetitive, brief coronary artery occlusions produce collateral development in experimental animals. This model causes coronary collateralization in a highly reproducible fashion, but the process is very labor intensive. We report the design and use of a fully automated hydraulic coronary occlusion device capable of producing repetitive coronary occlusions and enhancement of coronary collateral development in dogs.

METHODS: The device consists of analog electronics that allow adjustment of occlusion number, frequency, pressure and duration, and mechanical components responsible for the coronary occlusion. The motor and piston of the device are coupled to a chronically implanted hydraulic vascular occluder placed around the left anterior descending coronary artery (LAD) of dogs instrumented for measurement of systemic and coronary hemodynamics. One group of dogs (n = 6) underwent brief (2 min) LAD occlusions once per hour, eight times per day, 5 days/week for 3 weeks to stimulate collateral development (measured using radioactive microspheres). Another group of dogs (n = 6) that did not receive repetitive occlusions served as controls.

RESULTS: The device reproducibly produced repetitive LAD occlusions for the duration, frequency, and time interval initially programmed. A time-dependent increase in transmural collateral blood flow was observed in dogs undergoing repetitive occlusions using the device. Collateral blood flow was unchanged in dogs that did not undergo occlusions.

DISCUSSION: The automated occluder device reliably produces repetitive coronary occlusions and may facilitate further study of coronary collateral development in response to chronic myocardial ischemia.

Author List

Rys R, LaDisa JF Jr, Tessmer JP, Gu W, Kersten JR, Warltier DC, Pagel PS

Author

John F. LaDisa PhD Professor in the Pediatrics department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Animals
Collateral Circulation
Constriction
Coronary Circulation
Coronary Disease
Coronary Vessels
Disease Models, Animal
Dogs
Equipment Design
Models, Cardiovascular