Faculty Collaboration Database

Stress-activated protein kinase phosphorylation during cardioprotection in the ischemic myocardium. Am J Physiol Heart Circ Physiol 2001 Sep;281(3):H1184-92

Date

08/22/2001

Pubmed ID

11514286

DOI

10.1152/ajpheart.2001.281.3.H1184

Scopus ID

2-s2.0-0034829970 (requires institutional sign-in at Scopus site)   86 Citations

Abstract

Stress-activated protein kinases may be essential to cardioprotection. We assessed the role of p38 in an in vivo rat model of ischemia-reperfusion. Ischemic preconditioning (IPC) and the delta(1)-opioid receptor agonist 2-methyl-4aalpha-(3-hydroxyphenyl)-1,2,3,4,4a,5,12,12aalpha-octahydroquinolino [2,3,3-g]isoquinoline (TAN-67) significantly reduced infarct size (IS), expressed as a percentage of the area at risk (AAR), versus animals subjected only to 30 min of ischemia and 2 h of reperfusion (7.1 +/- 1.5 and 29.6 +/- 3.3 vs. 59.7 +/- 1.6%). The p38 antagonist SB-203580 attenuated IPC when it was administered before (34.0 +/- 6.9%) or after (25.0 +/- 3.8%) the IPC stimulus; however, it did not significantly attenuate TAN-67-induced cardioprotection (39.6 +/- 3.2). We also assessed the phosphorylation of p38 and c-jun NH(2)-terminal kinase (JNK) throughout ischemia-reperfusion in nuclear and cytosolic fractions. After either intervention, no increase was detected in the phosphorylation state of either enzyme in the nuclear fraction or for p38 in the cytosolic fraction versus control hearts. However, there was a robust increase in JNK activity in the cytosolic fraction immediately on reperfusion that was more pronounced in animals subjected to IPC or administered TAN-67. These data suggest that SB-203580 likely attenuates IPC via the inhibition of kinases other than p38, which may include JNK. The data also suggest that activation of JNK during early reperfusion may be an important component of cardioprotection.

Author List

Fryer RM, Patel HH, Hsu AK, Gross GJ

MESH terms used to index this publication - Major topics in bold

Animals
Coronary Vessels
Cytoprotection
Enzyme Inhibitors
Hemodynamics
Imidazoles
Ischemic Preconditioning, Myocardial
JNK Mitogen-Activated Protein Kinases
Male
Mitogen-Activated Protein Kinases
Myocardial Ischemia
Myocardium
Phosphorylation
Pyridines
Quinolines
Rats
Rats, Wistar
Receptors, Opioid, delta
Reperfusion Injury
p38 Mitogen-Activated Protein Kinases