Positional cloning of the major quantitative trait locus underlying lung tumor susceptibility in mice. Proc Natl Acad Sci U S A 2003 Oct 28;100(22):12642-7
Date
10/30/2003Pubmed ID
14583591Pubmed Central ID
PMC240671DOI
10.1073/pnas.2133947100Scopus ID
2-s2.0-0242331667 (requires institutional sign-in at Scopus site) 37 CitationsAbstract
Pulmonary adenoma susceptibility 1 (Pas1), located on chromosome 6, is the major locus affecting inherited predisposition to lung tumor development in mice. We have fine mapped the Pas1 locus to a region of approximately 0.5 megabases by using congenic strains of mice, constructed by placing the Pas1 region of chromosome 6 from A/J mice onto the genetic background of C57BL/6J mice. Systematic characterization of Pas1 candidates establishes the Las1 (lung adenoma susceptibility 1) and Kras2 (Kirsten rat sarcoma oncogene 2) genes as primary candidates for the Pas1 locus. Clearly, Kras2 affects lung tumor progression only, and Las1 is likely to affect lung tumor multiplicity.
Author List
Zhang Z, Futamura M, Vikis HG, Wang M, Li J, Wang Y, Guan KL, You MMESH terms used to index this publication - Major topics in bold
AdenomaAmino Acid Sequence
Animals
Base Sequence
Blotting, Northern
Chromosome Mapping
Ciona intestinalis
Cloning, Molecular
DNA Primers
Genetic Markers
Genetic Predisposition to Disease
Humans
Lung Neoplasms
Male
Mice
Mice, Inbred A
Mice, Inbred C57BL
Mice, Nude
Microsatellite Repeats
Molecular Sequence Data
Proto-Oncogene Proteins
Proto-Oncogene Proteins p21(ras)
Quantitative Trait Loci
Reverse Transcriptase Polymerase Chain Reaction
Sequence Alignment
Sequence Homology, Amino Acid
ras Proteins
