Faculty Collaboration Database

Evidence that renal arginine transport is impaired in spontaneously hypertensive rats. Am J Physiol Renal Physiol 2012 Jun 15;302(12):F1554-62

Date

03/24/2012

Pubmed ID

22442214

DOI

10.1152/ajprenal.00084.2011

Scopus ID

2-s2.0-84862589362 (requires institutional sign-in at Scopus site)   9 Citations

Abstract

Low renal nitric oxide (NO) bioavailability contributes to the development and maintenance of chronic hypertension. We investigated whether impaired l-arginine transport contributes to low renal NO bioavailability in hypertension. Responses of renal medullary perfusion and NO concentration to renal arterial infusions of the l-arginine transport inhibitor l-lysine (10 μmol·kg(-1)·min(-1); 30 min) and subsequent superimposition of l-arginine (100 μmol·kg(-1)·min(-1); 30 min), the NO synthase inhibitor N(G)-nitro-l-arginine (2.4 mg/kg; iv bolus), and the NO donor sodium nitroprusside (0.24 μg·kg(-1)·min(-1)) were examined in Sprague-Dawley rats (SD) and spontaneously hypertensive rats (SHR). Renal medullary perfusion and NO concentration were measured by laser-Doppler flowmetry and polarographically, respectively, 5.5 mm below the kidney surface. Renal medullary NO concentration was less in SHR (53 ± 3 nM) compared with SD rats (108 ± 12 nM; P = 0.004). l-Lysine tended to reduce medullary perfusion (-15 ± 7%; P = 0.07) and reduced medullary NO concentration (-9 ± 3%; P = 0.03) while subsequent superimposition of l-arginine reversed these effects of l-lysine in SD rats. In SHR, l-lysine and subsequent superimposition of l-arginine did not significantly alter medullary perfusion or NO concentration. Collectively, these data suggest that renal l-arginine transport is impaired in SHR. Renal l-[(3)H]arginine transport was less in SHR compared with SD rats (P = 0.01). Accordingly, we conclude that impaired arginine transport contributes to low renal NO bioavailability observed in the SHR kidney.

Author List

Rajapakse NW, Kuruppu S, Hanchapola I, Venardos K, Mattson DL, Smith AI, Kaye DM, Evans RG

MESH terms used to index this publication - Major topics in bold

Animals
Arginine
Biological Transport
Hemodynamics
Hypertension
Kidney
Lysine
Male
Nitric Oxide
Nitroprusside
Rats
Rats, Inbred SHR
Rats, Sprague-Dawley
Renal Circulation