Faculty Collaboration Database

Differentially expressed genes associated with mouse lung tumor progression. Oncogene 2002 Aug 22;21(37):5814-21

Date

08/13/2002

Pubmed ID

12173053

DOI

10.1038/sj.onc.1205422

Scopus ID

2-s2.0-0037158893 (requires institutional sign-in at Scopus site)   51 Citations

Abstract

To detect altered gene expression associated with mouse lung tumor progression, we compared the gene expression profile of lung adenocarcinomas with that of lung adenomas and normal lungs. Autoradiographic analysis showed that among the 588 genes surveyed, 152 genes were detected and the remaining 436 genes did not give any signals. A gene-specific semiquantitative reverse transcription polymerase chain reaction method was used to confirm the expression profile. A total of 29 genes was found to be differentially expressed in mouse lung tumors when compared to normal lungs. The pattern of expression, either underexpression or overexpression, was the same for 10 genes between adenocarcinomas and adenomas. Among them, seven genes were overexpressed, two genes were underexpressed and one gene was lost. Interestingly, 19 genes showed differential expression or increased incidence or difference in level of change between lung adenomas and adenocarcinomas, including Stat1, ADAP, IGFBP-6, PDGF-A, TGF-beta2, Int-3, VEGFR2, BAX, BAG-1, c-Jun, FasL, TRAIL, YB-1, CD31, Cdc42, B-raf, Rab-2, Abi-1, and ACE. These genes can be designated as candidate 'lung tumor progression' (LTP) genes because their expression changes may specifically affect lung tumor progression in mice. Further analyses of these candidate LTP genes may provide new leads for elucidation of lung tumor progression in mice.

Author List

Yao R, Wang Y, Lubet RA, You M

MESH terms used to index this publication - Major topics in bold

Adenocarcinoma
Adenoma
Animals
Apoptosis
Female
Lung
Lung Neoplasms
Mice
Oligonucleotide Array Sequence Analysis
Reverse Transcriptase Polymerase Chain Reaction