Detection of genetic alterations in mouse lung adenocarcinomas by two-dimensional gel electrophoresis. Exp Lung Res 2000 Dec;26(8):651-8
Date
02/24/2001Pubmed ID
11195462DOI
10.1080/01902140150216738Scopus ID
2-s2.0-0034521636 (requires institutional sign-in at Scopus site) 1 CitationAbstract
DNA from 14 mouse lung adenocarcinomas and 7 normal lungs were examined by 2-dimensional gel electrophoresis (2-DGE) for genetic alterations. 2-DGE profiles from tumor samples were compared with those profiles from normal lung tissues through a computer-assisted color overlay system. Compared to the profiles in normal lung DNA, 6 spots were amplified and 16 spots were partially reduced in their intensity in tumors. Two spots were detectable only in tumor tissues. These altered spots indicate genetic changes in mouse lung tumor development. The identification of these genetic alterations is probably important in understanding mouse lung carcinogenesis.
Author List
Liu J, Gu P, Bergman G, Kelloff GJ, Boone CW, You M, Wang YMESH terms used to index this publication - Major topics in bold
AdenocarcinomaAnimals
Chromosome Deletion
DNA Methylation
DNA, Neoplasm
Electrophoresis, Gel, Two-Dimensional
Gene Amplification
Image Processing, Computer-Assisted
Lung Neoplasms
Mice
Mice, Inbred C3H
Mice, Inbred CBA
