Deoxyglucose-resistant mutants of Neurospora crassa: isolation, mapping, and biochemical characterization. J Bacteriol 1989 Jan;171(1):53-8
Date
01/01/1989Pubmed ID
2521617Pubmed Central ID
PMC209552DOI
10.1128/jb.171.1.53-58.1989Scopus ID
2-s2.0-0024568866 (requires institutional sign-in at Scopus site) 51 CitationsAbstract
Neurospora crassa mutants resistant to 2-deoxyglucose have been isolated, and their mutations have been mapped to four genetic loci. The mutants have the following characteristics: (i) they are resistant to sorbose as well as to 2-deoxyglucose; (ii) they are partially or completely constitutive for glucose transport system II, glucamylase, and invertase, which are usually repressed during growth on glucose; and (iii) they synthesize an invertase with abnormal thermostability and immunological properties, suggesting altered posttranslational modification. All of these characteristics could arise from defects in the regulation of carbon metabolism. In addition, mutants with mutations at three of the loci lack glucose transport system I, which is normally synthesized constitutively by wild-type N. crassa. Although the basis for this change is not yet clear, the mutants provide a way of studying the high-affinity system II uncomplicated by the presence of the low-affinity system I.
Author List
Allen KE, McNally MT, Lowendorf HS, Slayman CW, Free SJMESH terms used to index this publication - Major topics in bold
3-O-MethylglucoseBiological Transport, Active
Chromosome Mapping
Chromosomes, Bacterial
Deoxy Sugars
Deoxyglucose
Drug Resistance, Microbial
Genotype
Glycoside Hydrolases
Kinetics
Methylglucosides
Mutation
Neurospora
Neurospora crassa
Recombination, Genetic
beta-Fructofuranosidase
