Models of gastric hyperalgesia in the rat. Am J Physiol Gastrointest Liver Physiol 2002 Sep;283(3):G666-76
Date
08/16/2002Pubmed ID
12181181DOI
10.1152/ajpgi.00001.2002Scopus ID
2-s2.0-0036720959 (requires institutional sign-in at Scopus site) 92 CitationsAbstract
Despite the prevalence of dyspepsia, nonhuman models for study of gastric hyperalgesia are limited. We thus characterized responses to gastric distension (GD) in the absence of and after two different gastric insults. A balloon was surgically placed into the stomach, and electromyographic responses to GD were recorded from the acromiotrapezius muscle at various times after balloon placement. Rats received either 20% acetic acid (HAc) or saline injections into the stomach wall or 0.1% iodoacetamide (IA) in drinking water. Responses to GD were monotonic with increasing distending pressure (10-80 mmHg) and were reproducible from days 3-14 after balloon implantation. Both HAc injection and IA ingestion led to increased responses to GD (i.e., gastric hyperalgesia), which, in the case of HAc, persisted for 60 days after HAc treatment. HAc injection produced ulcers in all treated animals; IA ingestion produced no lesions. Myeloperoxidase activity significantly increased after HAc but not saline injection or IA ingestion. In the awake, unrestrained rat, visceromotor responses to GD are quantifiable, reliable, and reproducible. Significantly enhanced responses to GD were apparent in two models of gastric insult, both of which may be useful for the study of the mechanisms of gastric hyperalgesia.
Author List
Ozaki N, Bielefeldt K, Sengupta JN, Gebhart GFMESH terms used to index this publication - Major topics in bold
Acetic AcidAnimals
Avoidance Learning
Behavior, Animal
Catheterization
Disease Models, Animal
Electromyography
Gastritis
Gastrointestinal Motility
Hyperalgesia
Iodoacetamide
Male
Rats
Rats, Sprague-Dawley
Reproducibility of Results
Stomach Diseases
Stomach Ulcer
