Serotonin and serotonin receptor expression in the aorta of carotid intact and denervated newborns. Respir Physiol Neurobiol 2002 Sep 04;132(3):253-64
Date
09/05/2002Pubmed ID
12208084DOI
10.1016/s1569-9048(02)00119-2Scopus ID
2-s2.0-0037019778 (requires institutional sign-in at Scopus site) 8 CitationsAbstract
Pharmacological blocking of serotonin (5-HT) 5A receptors abolishes aortic ventilatory chemosensitivity of carotid body denervated (CBD) piglets [J. Appl. Physiol. 92 (2002) 893]. Accordingly, the purpose of the present study was to determine whether 5-HT and 5-HT receptors exist at aortic sites that are chemosensitive after CBD. Aortas from CBD and sham CBD rats and piglets and from aortic denervated (AOD) and combined AOD+CBD piglets were harvested, sectioned and then studied using immunohistochemistry and western blot techniques. 5-HT immunoreactivity in piglets and rats was concentrated in the endothelium and sub-endothelial areas in several aortic regions studied, and in some areas also in the adventitia. At the aortic chemosensitive site (descending aorta in CBD piglets and the ascending aorta in CBD rats), the immunoreactivity was greater (P < 0.05) than in other aortic regions and greater than in other groups studied. The 5-HT(5a) receptor was expressed only at the chemosensitive sites and only in aortic innervated piglets. We conclude that the data from this and a previous study [J. Appl. Physiol. 92 (2002) 893] suggest that a serotonergic mechanism contributes to the aortic ventilatory chemoreflex after CBD.
Author List
Serra A, Brozoski D, Simeon T, Yi J, Bastasic J, Franciosi R, Jacobs ER, Forster HVAuthor
Hubert V. Forster PhD Professor in the Physiology department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Analysis of VarianceAnimals
Animals, Newborn
Aorta
Blotting, Western
Carotid Arteries
Denervation
Immunohistochemistry
Rats
Rats, Sprague-Dawley
Receptors, Serotonin
Serotonin
Swine
