Activity of bleomycin in iron- and copper-deficient cells. Biochem Pharmacol 1989 Dec 01;38(23):4273-82
Date
12/01/1989Pubmed ID
2480795DOI
10.1016/0006-2952(89)90526-1Scopus ID
2-s2.0-0024332086 (requires institutional sign-in at Scopus site) 13 CitationsAbstract
Three models were used to examine the requirement of bleomycin (Blm) for iron (Fe) to carry out its antitumor or cytotoxic activity. Mice were made iron deficient by dietary means. Animals with depressed iron stores in liver and low plasma and ascites fluid iron supported Ehrlich tumor growth as well as mice maintained on a control diet. Bleomycin was equally effective against this tumor in iron-deficient mice as it was against the tumor in iron-sufficient controls. Likewise, nutrient copper deficiency did not change the efficacy of the drug. Ehrlich cells in culture were treated with a non-growth inhibiting concentration of the chelating agent, 1,10-phenanthroline before or during their exposure to bleomycin. Again, the treated cells were as sensitive to drug as controls, despite the fact that this ligand reduces cellular iron and zinc and can extract iron from Fe(II)Blm. Lastly, it was demonstrated that iron-depleted Euglena gracilis cells growing at reduced rates were as sensitive to growth inhibition by bleomycin as control cells.
Author List
Lyman S, Taylor P, Lornitzo F, Wier A, Stone D, Antholine WE, Petering DHMESH terms used to index this publication - Major topics in bold
AnimalsAscites
Bleomycin
Carcinoma, Ehrlich Tumor
Cell Division
Cell Line
Copper
Diet
Euglena gracilis
Female
Iron
Iron Chelating Agents
Mice
Phenanthrolines
Tumor Cells, Cultured
