Oxidized products of linoleic acid stimulate adrenal steroidogenesis. Endocr Res 2002 Nov;28(4):325-30
Date
01/18/2003Pubmed ID
12530633DOI
10.1081/erc-120016804Scopus ID
2-s2.0-0036942824 (requires institutional sign-in at Scopus site) 25 CitationsAbstract
Adrenal steroidogenesis is under complex control, and clinical observations suggest that not all regulators have been identified. We postulated that fatty acid oxidation products found in the diet or formed in the body could affect steroidogenesis. Linoleic acid is a prominent constituent of animal fat and is readily oxidized. We found that several products of linoleic acid oxidation affect production of aldosterone and corticosterone by isolated cells from rat adrenals. We characterized one linoleic acid derivative by gas chromatography/mass spectrometry. It is 12,13-epoxy-9-oxo-10(trans)-octadecenoic acid ("EKODE"). At concentrations between 1 and 30 microM, EKODE stimulated production of aldosterone by zona glomerulosa cells, but at concentrations above 50 microM, it was inhibitory. In zona fasciculata cells, EKODE stimulated corticosterone production at concentrations of 5 microM or greater, and there was no evidence of inhibition at high concentrations. Stimulation of steroidogenesis was observed after 15 min of incubation and continued for at least 2 hrs. The potential relevance of our findings to the hypertension of obesity is discussed.
Author List
Goodfriend TL, Ball DL, Raff H, Bruder ED, Gardner HW, Spiteller GAuthor
Hershel Raff PhD Professor in the Academic Affairs department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AldosteroneAnimals
Corticosterone
Dose-Response Relationship, Drug
Linoleic Acid
Oleic Acids
Osmolar Concentration
Oxidation-Reduction
Protein Isoforms
Rats
Zona Fasciculata
Zona Glomerulosa
