Characterization of a murine NKT cell tumor previously described as an acute myelogenous leukemia. Leuk Lymphoma 2002 Aug;43(8):1637-44
Date
10/29/2002Pubmed ID
12400607DOI
10.1080/1042819021000002974Scopus ID
2-s2.0-0036020969 (requires institutional sign-in at Scopus site) 20 CitationsAbstract
C1498 is an atypical myeloid leukemia that originated in a C57BL/6 mouse and has been used as a model for acute myelogenous leukemia. In studies of the immune response to C 1498, we found that this tumor contained mRNA encoding the canonical NKT cell receptor Vbeta8.2-Valpha14Jalpha281. Although cell-surface phenotypic analysis showed C1498 to be negative for NK1.1, it expressed several other molecules associated with NKT cell populations, such as DX5, CDld, CD69, CD44, CD45RB and B220. RT-PCR demonstrated that C1498 contained CD3epsilon mRNA transcripts, but message was not found for CD4, CD8alpha, or CD8beta. This indicates that C1498 falls within the double negative (CD4-CD8-) NKT cell lineage. RNase protection analysis showed that C1498 expressed mRNA for IL-2, IL-15, and macrophage migration inhibitory factor (MIF). These findings suggest that C1498 should be re-classified as a NKT cell leukemia with atypical myeloid features. It may, therefore, be a novel cell line in which to study NKT cell development and serve as a model for human NKT cell malignancies.
Author List
LaBelle JL, Truitt RLMESH terms used to index this publication - Major topics in bold
AnimalsCD3 Complex
CD4 Antigens
CD8 Antigens
Cytokines
Immunophenotyping
Killer Cells, Natural
Leukemia, Myeloid, Acute
Mice
Mice, Inbred C57BL
RNA, Messenger
Receptors, Antigen, T-Cell
Receptors, Antigen, T-Cell, alpha-beta
Tumor Cells, Cultured
