In vitro differentiation of quail neural crest cells into sensory-like neuroblasts. Brain Res 1988 Mar 01;467(1):69-83
Date
03/01/1988Pubmed ID
2452002DOI
10.1016/0165-3806(88)90068-5Scopus ID
2-s2.0-0023832254 (requires institutional sign-in at Scopus site) 13 CitationsAbstract
This study shows that quail neural crest cells can differentiate in vitro into sensory-like neuroblasts. The putative sensory neuroblasts were large and spherical, possessing large diameter, bipolar or pseudo-unipolar, long processes that lacked multiple varicosities characteristic of autonomic neurons. They bound HNK-1, a monoclonal antibody against a cell surface epitope expressed by early neural crest cells but not by young neural tube-derived cells. Many of the sensory-like neuroblasts had substance P (SP)-like immunoreactivity. Some exhibited histochemical carbonic anhydrase activity; carbonic anhydrase is shown in this study to stain a subpopulation of spinal sensory neurons in adult quail and embryos 9 days and older, whereas ventral root axons and neurons in sympathetic ganglia are non-reactive at all ages. Double staining indicated that unlike the multipolar neuroblasts developing in the same cultures, SP-like immunoreactive neuroblasts do not contain detectable levels of tyrosine hydroxylase or dopamine-beta-hydroxylase. Finally, the neuronal nature of the cultured sensory-like neuroblasts was further documented by double labeling for antibodies against the 68 kDa neurofilament polypeptide and substance P.
Author List
Sieber-Blum M, Kumar SR, Riley DAMESH terms used to index this publication - Major topics in bold
AnimalsAntibodies, Monoclonal
Carbonic Anhydrases
Cell Differentiation
Cells, Cultured
Chick Embryo
Fibronectins
Immunohistochemistry
Intermediate Filament Proteins
Nerve Growth Factors
Neural Crest
Neurofilament Proteins
Neurons, Afferent
Quail
Substance P
