Acute effects of prostaglandin E1 and E2 on vascular reactivity and blood flow in situ in the chick chorioallantoic membrane. Prostaglandins Leukot Essent Fatty Acids 2012;87(2-3):79-89
Date
08/04/2012Pubmed ID
22858445Pubmed Central ID
PMC3839254DOI
10.1016/j.plefa.2012.07.002Scopus ID
2-s2.0-84865387236 (requires institutional sign-in at Scopus site) 4 CitationsAbstract
The chick chorioallantoic membrane (CAM) subserves gas exchange in the developing embryo and shell-less culture affords a unique opportunity for direct observations over time of individual blood vessels to pharmacologic interventions. We tested a number of lipids including prostaglandins PGE(1&2) for vascular effects and signaling in the CAM. Application of PGE(1&2) induced a decrease in the diameter of large blood vessels and a concentration-dependent, localized, reversible loss of blood flow through small vessels. The loss of flow was also mimicked by misoprostol, an agonist for 3 of 4 known PGE receptors, EP(2-4), and by U46619, a thromboxane mimetic. Selective receptor antagonists for EP(3) and thromboxane each partially blocked the response. This is a first report of the effects of prostaglandins on vasoreactivity in the CAM. Our model allows the unique ability to examine simultaneous responses of large and small vessels in real time and in vivo.
Author List
Harland DR, Lorenz LD, Fay K, Dunn BE, Gruenloh SK, Narayanan J, Jacobs ER, Medhora MMESH terms used to index this publication - Major topics in bold
15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic AcidAlprostadil
Animals
Biphenyl Compounds
Blood Vessels
Bridged Bicyclo Compounds, Heterocyclic
Chick Embryo
Chorioallantoic Membrane
Dibenz(b,f)(1,4)oxazepine-10(11H)-carboxylic acid, 8-chloro-, 2-acetylhydrazide
Dinoprostone
Dose-Response Relationship, Drug
Estradiol
Estrogens
Fatty Acids, Unsaturated
Hydrazines
Microsomes
Misoprostol
Prostaglandin Antagonists
Rats
Receptors, Prostaglandin
Receptors, Thromboxane
Vasoconstriction
Vasoconstrictor Agents
Xanthones
alpha-Linolenic Acid
