Faculty Collaboration Database

Realistic modeling of the illumination point spread function in confocal scanning optical microscopy. J Opt Soc Am A Opt Image Sci Vis 2010 Feb 01;27(2):295-302

Date

02/04/2010

Pubmed ID

20126241

DOI

10.1364/JOSAA.27.000295

Scopus ID

2-s2.0-76749163839 (requires institutional sign-in at Scopus site)   107 Citations

Abstract

In this paper we present a rigorous and general theoretical model for the illumination point spread function of a confocal microscope that correctly reproduces the optical setup. The model uses vectorial theory and assumes that monochromatic light with a Gaussian intensity distribution (such as from a laser or a single-mode fiber) is focused by a microscope objective with high numerical aperture and passes through stratified media on its way to the sample. This covers the important practical case of illumination through up to three layers, which is the situation most commonly encountered in biological microscopy (immersion oil, glass coverslip, aqueous sample medium). It also accounts for objectives that are corrected for a certain coverslip thickness and refractive index but operated under non-design conditions. Furthermore, illumination with linearly, circularly, or elliptically polarized light is covered by introducing a Babinet-Soleil compensator into the beam path. The model leads to a set of analytical equations that are readily evaluated. Two-dimensional intensity distributions for particular cases of interest are presented and discussed.

Author List

Nasse MJ, Woehl JC

Author

Jorg C. Woehl in the Chemistry and Biochemistry department at University of Wisconsin - Milwaukee

MESH terms used to index this publication - Major topics in bold

Algorithms
Computer Simulation
Diagnostic Imaging
Equipment Design
Light
Microscopy, Confocal
Models, Statistical
Models, Theoretical
Normal Distribution
Optics and Photonics