Faculty Collaboration Database

Molecular characterization of the Azotobacter vinelandii recF gene. Gene 1995 Aug 30;162(1):47-51

Date

08/30/1995

Pubmed ID

7557415

DOI

10.1016/0378-1119(95)00307-r

Scopus ID

2-s2.0-0029080119 (requires institutional sign-in at Scopus site)   3 Citations

Abstract

The recF gene from Azotobacter vinelandii (Av) has been cloned by complementation in an Escherichia coli (Ec) recF mutant. The sequence of 1568 bp has been determined and analyzed. It showed an open reading frame of 1092 nt coding for a 364-amino-acid (aa) polypeptide. The comparison of the deduced aa sequence of the recF of Av with those of other bacteria has elicited the presence of the four conserved domains thought to be essential for RecF function. A transcriptional fusion of a DNA fragment containing the promoter sequence of recF with the lacZ gene of Ec was constructed and 3-4-fold enhancement of promoter activity was observed upon UV induction.

Author List

Badran H, Venkatesh TV, Kunnimalaiyaan M, Sharma N, Das HK

MESH terms used to index this publication - Major topics in bold

Amino Acid Sequence
Azotobacter vinelandii
Bacterial Proteins
Base Sequence
DNA-Binding Proteins
Escherichia coli
Escherichia coli Proteins
Gene Expression Regulation, Bacterial
Genes, Bacterial
Genes, Reporter
Genomic Library
Molecular Sequence Data
Open Reading Frames
Promoter Regions, Genetic
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Ultraviolet Rays