Faculty Collaboration Database

Placental expression of glucose transporter proteins 1 and 3 in growth-restricted fetal rats. Am J Obstet Gynecol 1999 Apr;180(4):1017-23

Date

04/16/1999

Pubmed ID

10203672

DOI

10.1016/s0002-9378(99)70675-7

Scopus ID

2-s2.0-0032931146 (requires institutional sign-in at Scopus site)   18 Citations

Abstract

OBJECTIVE: The purpose of this study was to determine the effect of intrauterine growth restriction on the placental expression of glucose transporter proteins.

STUDY DESIGN: Intrauterine growth restriction was induced by bilateral uterine artery ligation in the pregnant rat at a gestational age of 19 days (term is 21.5 days). Maternal rats were killed and fetuses were delivered by hysterotomy on gestational days 20 and 21. Control fetuses from mothers that had been subjected to a sham operation were studied simultaneously. Glucose transporter protein 1 and glucose transporter protein 3 messenger ribonucleic acid was quantified by reverse transcriptase-polymerase chain reaction amplification. Glucose transporter protein 1 and glucose transporter protein 3 densities in placental membranes were also assessed by Western blotting and by immunohistochemical analysis.

RESULTS: Glucose transporter protein 1 messenger ribonucleic acid, expressed as a multiple of the matched sham control value, was unchanged on both days 20 and 21 of gestation. Glucose transporter protein 3 messenger ribonucleic acid was also unchanged. Western blotting demonstrated no change in expression of glucose transporter protein 1 or glucose transporter protein 3 on either day 20 or 21 of gestation. Immunohistochemical staining patterns for glucose transporter protein 1 and glucose transporter protein 3 on the syncytiotrophoblastic membranes were similar between the growth-restricted group and the sham control group.

CONCLUSION: Placental expression of glucose transporter proteins in the pregnant rat is unchanged with uteroplacental insufficiency.

Author List

Reid GJ, Lane RH, Flozak AS, Simmons RA

MESH terms used to index this publication - Major topics in bold

Animals
Blood Glucose
Blotting, Western
Female
Fetal Blood
Fetal Growth Retardation
Gene Expression Regulation, Developmental
Immunohistochemistry
Insulin
Monosaccharide Transport Proteins
Placenta
Pregnancy
RNA, Messenger
Rats
Rats, Sprague-Dawley
Reverse Transcriptase Polymerase Chain Reaction