Gankyrin, a biomarker for epithelial carcinogenesis, is overexpressed in human oral cancer. Anticancer Res 2011 Sep;31(9):2683-92
Date
08/27/2011Pubmed ID
21868508Scopus ID
2-s2.0-79959434779 (requires institutional sign-in at Scopus site) 34 CitationsAbstract
UNLABELLED: Little is known about the potential involvement of the oncoprotein gankyrin in human oral cancer progression. In this study, the levels of gankyrin mRNA and protein expression were assessed in human oral epithelial cell lines, at-risk normal oral tissues, premalignant oral lesions, and primary oral squamous cell carcinomas (OSCCs).
MATERIALS AND METHODS: Biopsies included 6 oral epithelial cell lines, 32 OSCC specimens for qRT-PCR analysis, 27 OSCC specimens and 12 premalignant oral lesions for immunohistochemical analysis.
RESULTS: Gankyrin was overexpressed in all tested oral epithelial cell lines and the majority of OSCC specimens (32/32 (100%) and 21/27 (71%) at the mRNA and protein levels, respectively). Moreover, 6/12 of premalignant oral lesions overexpressed gankyrin protein.
CONCLUSION: Gankyrin overexpression is a prevalent event in human oral cancer and occurs during the early stages of oral carcinogenesis, thus being a viable therapeutic or chemopreventive target in oral cancer.
Author List
Li J, Knobloch TJ, Kresty LA, Zhang Z, Lang JC, Schuller DE, Weghorst CMMESH terms used to index this publication - Major topics in bold
AdultAged
Base Sequence
Biomarkers, Tumor
Carcinoma, Squamous Cell
Cell Transformation, Neoplastic
DNA Primers
Female
Humans
Male
Middle Aged
Mouth Neoplasms
Proteasome Endopeptidase Complex
Proto-Oncogene Proteins
Reverse Transcriptase Polymerase Chain Reaction
