The effects of reactive species on the tumorigenic phenotype of human head and neck squamous cell carcinoma (HNSCC) cells. Anticancer Res 2007;27(6B):3819-27
Date
01/30/2008Pubmed ID
18225538Pubmed Central ID
PMC2408378Scopus ID
2-s2.0-37549053228 (requires institutional sign-in at Scopus site) 10 CitationsAbstract
Sustained inflammation up-regulates the reactive species (RS) generating enzymes inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). While clinical data show that levels of iNOS and COX-2 are increased in epithelium during the transformation of dysplasia to overt head and neck squamous cell carcinoma (HNSCC), the mechanisms by which their overexpression contributes to HNSCC development have not been completely delineated. This study assessed the effects of RS on parameters associated with the HNSCC tumorigenic phenotype inclusive of activation of NF-kappaB (in situ immunostaining and reporter assay) and production of proinflammatory and proangiogenic proteins (ELISA analyses). Our data, which show both reactive oxygen and nitrogen species activated NF-kappaB, and that all RS donors evaluated increased HNSCC cellular production of vascular endothelial growth factor, IL-8 and epidermal growth factor receptor proteins, imply inflammation associated RS promote HNSCC by their abilities to modulate intracellular signaling and affect gene expression.
Author List
Bradburn JE, Pei P, Kresty LA, Lang JC, Yates AJ, McCormick AP, Mallery SRMESH terms used to index this publication - Major topics in bold
Carcinoma, Squamous CellCell Line, Tumor
Cell Survival
ErbB Receptors
Head and Neck Neoplasms
Humans
I-kappa B Proteins
Interleukin-8
NF-kappa B
Oxidation-Reduction
Reactive Nitrogen Species
Reactive Oxygen Species
Vascular Endothelial Growth Factor A
