Activated protooncogenes in human lung tumors from smokers. Proc Natl Acad Sci U S A 1991 Feb 15;88(4):1085-9
Date
02/15/1991Pubmed ID
1996309Pubmed Central ID
PMC50961DOI
10.1073/pnas.88.4.1085Scopus ID
2-s2.0-0026030262 (requires institutional sign-in at Scopus site) 74 CitationsAbstract
Fourteen primary human lung tumor DNAs from smokers were analyzed for transforming activity by two DNA transfection assays. Activated protooncogenes were detected in 3 of 11 tumor DNAs by the NIH 3T3 focus assay, whereas activated protooncogenes were detected in 11 of 13 tumor DNAs by the NIH 3T3 cotransfection-nude mouse tumorigenicity assay. K- or NRAS genes activated by point mutation at codons 12 or 61 were detected in a large cell carcinoma, a squamous cell carcinoma, and 5 adenocarcinomas. An HRAS oncogene activated by a different mechanism was detected in an epidermoid carcinoma. One adenocarcinoma was found to contain an activated RAF gene. Two unidentified transforming genes were detected in a squamous cell carcinoma DNA and two adenocarcinoma DNAs. Eight of 10 lung adenocarcinomas that had formed metastases at the time of surgery were found to contain RAS oncogenes. No significant increase in metastasis was observed in the lung adenocarcinomas that contained one or more 6-kilobase EcoRI alleles of the LMYC gene. Overall, 12 of 14 (86%) of the lung tumor DNAs from smokers were found to contain activated protooncogenes. RAS oncogenes appear to play a role in the development of metastases in lung adenocarcinomas.
Author List
Reynolds SH, Anna CK, Brown KC, Wiest JS, Beattie EJ, Pero RW, Iglehart JD, Anderson MWMESH terms used to index this publication - Major topics in bold
AdenocarcinomaAnimals
Cell Line
Cell Transformation, Neoplastic
DNA
DNA, Neoplasm
Gene Expression Regulation, Neoplastic
Humans
Lung Neoplasms
Mice
Mice, Nude
Neoplasm Transplantation
Nucleic Acid Hybridization
Polymerase Chain Reaction
Proto-Oncogenes
Smoking
Transfection
Transplantation, Heterologous
